Biofilm production ability and associated characteristics of Streptococcus agalactiae isolates from companion animals and humans

Objective We evaluated biofilm production ability (BPA) of Streptococcus agalactiae isolates from companion animals/humans and clarified the relationship between BPA populations other microbiological features. Methods Companion animal-/human-origin were collected with host information. measured using crystal violet staining, via virulence-associated gene profiling (hylB-pavA-pilB-spb1-srtC1-brpA), capsular genotyping, multilocus sequence typing, antimicrobial resistance (AMR) phenotyping/genotyping. Significant difference in different hosts was assessed. analyzed association virulence genotypes, types/clonal complexes, AMR phenotypes/genotypes. Inhibitory effect berberine on evaluated. Results Five, twenty-six, twenty-six belonged to strong, moderate, weak producers, whereas seventeen showed no production. defined or producers as producer group (n = 57) conduct a comparative analysis non-producer populations. There significant correlation population vaginal specimen. found associations presence (57.9%) pilB (70.6%) spb1. phenotypes/genotypes (except for minocycline). confirmed inhibitory at sub-minimum concentrations (MICs) against type strain BPA. Conclusion Our observations suggest that S. harboring is more capable producing biofilms, sub-MICs